Image 1.19 Manual

Image Version 1.19 2 Introduction Menus 3 File 8 Edit 11 Options 15 Enhance 18 Analyze 23 Video 24 Text 25 Windows 25 Using the Tools Managing Windows 29 Image Windows 29 Gray Map 30 LUT 30 Histogram 31 Results Techniques 32 Using Selections 33 Analyzing Gels 34 Redirected Sampling 35 Using the Option Key 36 Questions and Answers 37 Using Flatbed Scanners Appendices 38 A. Frame Grabber Cards 39 B. TV Camera and Light Box 40 C. Alternate Programs 41 D. File Formats 42 E. Program Limitations 43 F. Shortcuts 44 G. New Versions and Bug Reports Introduction Image is a public domain program for the Macintosh II for doing digital image processing and analysis. It can acquire, enhance, measure, edit, animate, print and pseudocolor images. It reads and writes TIFF and PICT files, providing compatibility with many other Macintosh applications, including programs for scanning, processing, editing, publishing, and analyzing images. It supports many standard image processing functions, including histogram equalization, contrast enhancement, density profiling, smoothing, sharpening, edge detection, and noise reduction. Spatial convolutions, with user defined kernels up to 63x63, are supported. Image can be used to measure lengths and x-y coordinates, and compute the average density and area of user defined regions of interest. Length and area measurements results can be calibrated to provide real world values. Density calibration can be done against radiation or optical density standards using any user specified units. The user can select from any of eight different curve fitting methods for generating calibration curves. It provides MacPaint-like editing of color and grayscale images, including the ability to draw lines, rectangles, ovals and text. It can flip, rotate, invert and scale selections. It supports multiple windows and 8 levels of magnification. All editing, filtering, and measurement functions operate at any level of magnification and are undoable. It uses digital halftoning to print images on PostScript printers and Floyd-Steinberg dithering for printing on non-PostScript printers. It supports either the Data Translation QuickCapture card or Scion Image Capture 2 card for digitizing images using a TV camera. Acquired images can be shading corrected and frame averaged. Image is written in Lightspeed Pascal from Symantec Corporation, and the source code is available. The program can be easily ported to other Pascal compilers for the Macintosh, such as MPW Pascal or TML Pascal. System Requirements For full operation, Image requires a Mac II(x, cx, or ci) with at least 2 megabytes of memory, but 4 megabytes, or more, is recommended for doing animation, for simultaneously displaying more than a handful of pictures, or for running under MultiFinder. Image also requires an 8-bit video card capable of displaying 256 c…