Yes I was using recent PC formatted cartridges, so they were not recognized by the Mac without the Iomega Init in the System folder, but were easily re-formatted with Iomega's 4.2 Zip Tools under System 6.
As for the updated driver problem, I thought the main issue was having to reformat the disk with the 4.2 driver Tools, meaning back it up and re-initialize, a real pain either way. All of my old Zip disks were updated to newer drivers at some point back in the day before I knew about this problem. However, I have since successfully restored all of them to bootable 4.2 drivers using the 4.2 Zip Tools alone (under OS 8.6 no less).
Also, I presume you formatted the Zip 250 disk with a third party utility and not the Zip tools which would require System 7 and likely not be recognized by the Plus?
This may be of some help with third party formatters from Michael Peters's defunct website:
This app uses the SIOUX console library
Choose 'Quit' from the file menu to quit.
Use fake disk names (/dev/scsi.; i.e. /dev/scsi0.1, /dev/scsi1.3, etc.).
Top level command (? for help): l /dev/scsi0.5
finding devices .......
Partition map (with 512 byte blocks) on '/dev/scsi0.5' (/dev/sdb)
#: type name length base ( size )
1: Apple_partition_map 80 @ 1
2: Iomega_DiskInfo 10 @ 81
3: Apple_Driver* 400 @ 91
4: Apple_HFS 196106 @ 491 ( 95.8M)
5: Iomega_backup 1 @ 196597
6: Iomega_DiskInfo 10 @ 196598
Device block size=512, Number of Blocks=196608 (96.0M)
DeviceType=0x0, DeviceId=0x0
Drivers-
1: @ 91 for 4, type=0x1
Top level command (? for help):